Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
Abstract Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a Pipe Tools challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing in